Isotope-labeled Metabolites

Metabolites are the products and intermediates of cellular metabolism. Metabolites have a multitude of functions, including energy conversion, signaling, epigenetic influence, and cofactor activity. Metabolites cover a multitude of classes, including carnitine, acylcarnitines, drug standards, flavonoids, organic acids immunosuppressants, neurotransmitters, nucleic acids and derivatives, amino acids, lipids and vitamins. When one or more atoms of metabolites are replaced by the stable isotopes of that atom, that is stable isotope-labeled metabolites. Stable isotope-labeled metabolites provide flexibility and ease of use for multiple research areas based on MS-based metabolomics, such as disease diagnosis, medical research and development, nutritional food science, toxicology, environmental science, botanical studies.

Applications

Disease diagnosis: As intermediates and end products of biological processes in human body, metabolites are important indicators of normal physiological process and pathological process. Studying the changes of metabolites in body fluids and tissues can help to understand metabolic pathways and disease mechanisms and contribute to the diagnosis, treatment and prognosis of diseases. However, the content of metabolites in complex biological samples is generally low, and the matrix interferes with the analysis and determination greatly. So, it is difficult to achieve the ideal detection requirements by direct analysis. Based on stable isotope-labeled metabolites as internal standard in isotope dilution method can obtain the accurate and reliable absolute quantitative analysis results, which will be lay a strong foundation for predicting the progress of the disease, understanding the body metabolism pathway and exploring disease pathogenesis.

Drug development: The knowledge of the metabolic behavior of new chemical entities in drug development is one of the key prerequisites for their approval by regulative agencies. The stable isotope-labeled metabolites can then be used as internal standards for the quantification of drug metabolism in metabolic, toxicological, and pharmacokinetic studies to fully elucidate the metabolic behavior of new chemical drug entities and ensure their safe.

Food safety: Drug residue has become an important factor affecting the quality and safety of aquatic products. Drugs will be rapidly metabolized in animals, therefore, in aquatic product quality monitoring, the drug metabolites should be detected. Based on this, the establishment of accurate, reliable and sensitive quantitative and qualitative methods of drugs metabolites has far-reaching significance in ensuring the safety of aquatic products. Using stable isotope-labeled drug metabolites as internal standard can not only analyze drug metabolites in different states, but also improve the accuracy of quantitative analysis, which can meet the requirements of monitoring drug metabolites in food of animal origin.

Others: In addition, stable isotope-labeled metabolites are widely used in toxicology, environmental science, botany and other fields closely related to human health.